Advanced Neurogenomics

2019-2020

Course Objective

This course aims to introduce modern techniques in the integrated
Neurosciences via hands-on experience. The course illustrates
experimental approaches to seek causal relationship between molecular
events, such as changes in gene-expression, gene-mutations,
protein-protein interactions, and effects of such events on the
functioning of cells, networks and the whole organism. The use of these
techniques is embedded in short research projects and typically covers a
number of steps: first clone a construct (a mutant protein, an
over-expression construct), than culture and tranfect a cell with this
construct and finally analyze a functional consequences of this
modification using functional assays. Alternatively, research projects
may also start with the intact brain and then work down to the molecular
networks of proteins and genes, for instance using proteomics
and mRNA expression profiling. Although centered around genomics, many
functional assays are offered, from electronmicroscopy and protein
chemistry to life cell imaging, electrophysiology and behavioural
analyses. Various tools and technologies will be explained in supporting
lectures and used in order to answer questions related to
the function of genes in the nervous system and how they contribute to
higher order processes important for functioning of circuitry or
behavior.

End terms:
Profound insight and experience with neurogenomic techniques that are
used during the course
Adequate design, execution, and interpretation of neurogenomics
experiments and conceptualisation of new working models (empirical
cycle)
Adequate oral presentation (2 oral presentations)

Course Content

This is a practical course. You will be executing a small research
project aimed at illustrating today's research into gene-function
relations and finding causal relationships between molecular events and
functional consequences at the cellular/network level. The experiments
involve gene expression analysis (transcripts, proteins), and genotypic
and phenotypic analysis (genotyping, cell biology, behavior).
Theoretical underpinning of concepts and methods will be achieved by
using examples from recent literature.

Teaching Methods

Lectures, discussion of papers, demonstrations, practicals
16x8h practicals, presentations and discussion
4x8h self study

Method of Assessment

Examination and presentations of the work performed. Evaluation on the
basis of 2 oral presentations by the students and their performance
during the practical work and during (plenary) discussions.

Entry Requirements

Successful completion of the course Neurogenomics (MN 1st year) is a
requirement for enrolment in
this course.

The number of participants is limited to 20. In case
of over-subscription, priority will be given to students in the
Fundamental Neuroscience track of the MSc Neurosciences programme.

Literature

Articles form journals that published in the last couple of months (2-4
per student)
Purves Neuroscience (5th edition) as reference book
Literature can be studied during the course and will be provided at the
start of the course.

Additional Information

E-mail: matthijs@cncr.vu.nl

General Information

Course Code AM_470717
Credits 6 EC
Period P1
Course Level 500
Language of Tuition English
Faculty Faculty of Science
Course Coordinator prof. dr. M. Verhage
Examiner prof. dr. M. Verhage
Teaching Staff prof. dr. M. Verhage

Practical Information

You need to register for this course yourself

Last-minute registration is available for this course.

Teaching Methods Practical