Dynamics of Biomolecules and Cells

2018-2019

Course Objective

Life is, by its very definition, a dynamic quantity. In this course an
overview is given of current scientific research fields that study the
dynamic processes that take place in biomolecules, membranes and cells
and are indispensable for biological functionality. This course aims to
highlight the 'what', 'why' and 'how' of these research fields: what are
the established and new biological insights, why are these insights
important, and how are these results being obtained.

Learning objectives
* Understand the physics underlying the biophysical research methods
featured in this course
* Be able to explain how these methods can be exploited to address
specific biological problems and identify strengths and weaknesses of
methods in biological context
* Critically reflect on your understanding of methods application in
assigned literature and formulate relevant questions
* Analyze scientific literature that uses the discussed methodologies to
addresses biological problems and reflect on suitability of used methods
* Communicate and present your literature analysis and critically
reflect on analyses by other students

Course Content

The significance of small movements to large-scale and slow
reorganizations are being discussed as well the experimental techniques
employed.
- DNA processing and dynamics (techniques: optical tweezers, AFM,
tethered particle motion, magnetic tweezers). DNA structure and
stability, DNA/RNA polymerase, DNA architectural proteins, DNA repair.
- Protein dynamics (techniques: ultrafast spectroscopy, Infrared and
Raman spectroscopy, single-molecule fluorescence). Photoactive proteins,
light-driven enzymes, Motor proteins, optogenetics.
- Membrane dynamics and remodeling (techniques AFM, single molecule,
electron microscopy). Photosynthesis, crowding and membrane protein
diffusion, Neuroimaging.
-superresolution microscopy

Teaching Methods

Lectures, guest lectures, literature essay and assignments, oral
literature presentation

Method of Assessment

- Literature assignments
- Essay (literature or research proposal)
- Oral literature presentation

Entry Requirements

BSc. Physics, BSc. Medical Natural Sciences, BSc Chemistry or comparable

Literature

Notes, handouts and papers.

Target Audience

mMNS-PoL, mPhys-LSBP, mPhys-PLH

General Information

Course Code X_422583
Credits 6 EC
Period P4
Course Level 400
Language of Tuition English
Faculty Faculty of Science
Course Coordinator dr. ir. I. Heller
Examiner dr. ir. I. Heller
Teaching Staff dr. ir. I. Heller
prof. dr. J.T.M. Kennis

Practical Information

You need to register for this course yourself

Last-minute registration is available for this course.

Teaching Methods Lecture
Target audiences

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