Proteomics in Biomedical Research

2018-2019

Course Objective

Function and structure of cells depend on the composition of proteins.
During pathological conditions, the expression of proteins is altered
leading to impaired function/structure of cells. Apart from changes in
expression level, post-translational protein changes occur as a result
of altered signaling pathways. The large-scale analysis of proteins and
their quantitative changes in health and disease, a research field
called proteomics, may provide candidate biomarkers and targets for
therapeutic interventions.

This proteomics course consists of one week of theory (lectures and
literature study) and one week of practice in the lab. Together this
will provide a solid basis for the understanding of what proteomics is
about, how its central technique, mass spectrometry, can be used for
global protein identification and quantification, and what
biomedical/clinical questions can be answered using an appropriate
experimental design. In the second week, students will get hands-on
experience with a real proteomics experiment and the generated data will
be used to illustrate what bioinformatics analyses can be done to enable
biological insight of large scale data.

Course Content

Protein identification by tandem mass spectrometry and database
searching;
Gel electrophoresis and mass spectroscopy techniques to quantify
isoform expression and the nature and extent of post translational
modifications;
Data mining: placing large scale protein expression data in a
biological context (network analysis).

Method of Assessment

Written exam

Literature

Syllabus including relevant articles

Additional Information

Minimum number of participants: 5, maximum: 12
For optimal participation, basic knowledge of molecular and cellular
biology is needed.

Contact:
Prof. dr. Connie R. Jimenez
Head OncoProteomics Laboratory
Department of Medical Oncology, VUmc Cancer Center Amsterdam
e-mail: c.jimenez@vumc.nl
Website: www.oncoproteomics.nl

Custom Course Registration

Students can register for this course and examinations via vunet.vu.nl (under My study, register for courses and exams). The general VU registration rules apply. Information on registration deadlines can be found in VUnet. Please note that the general VU rules are strict, both for booking of the classes and (resit-)exams.

Explanation Canvas

A CANVAS course is available for this module. Students will be
enrolled automatically when they register for the module via VUnet. The
lecturers will post information such as presentations slides and
literature links in the course.

General Information

Course Code M_CPROTBI09
Credits 3 EC
Period P3+4+5
Course Level 500
Language of Tuition English
Faculty VUmc School of Medical Sciences
Course Coordinator dr. C.R. Jimenez
Examiner prof. dr. J. van der Velden
Teaching Staff

Practical Information

You need to register for this course yourself

Teaching Methods Lecture, Study Group
Target audiences

This course is also available as: