Course ObjectiveFinal attainment:
The student knows:
• Principles and applications of protein over-expression, purification,
structure, function, and inhibition as well as the function of
antibiotics as protein inhibitors.
The student can:
• Apply protein bio-chemistry methods (protein over-expression, affinity
chromatography, spectro-photometry, fluorescence, protein labeling
methods, gel electrophoresis, activity tests).
Course ContentThe course consists of a mixture of lectures, practicals, computer
sessions and individual study. We will cover
concepts/methods/techniques that you can use to study a broad range of
relevant questions, e.g:
• How can I produce a protein using bacteria?
• How can I purify a protein?
• How can I investigate structure and function of a protein?
• How can I predict structure and function of a protein?
• How do antibiotics work as protein inhibitors?
• How can I design my experimental strategy?
• Which factors I have to think about to make my experiment
Teaching MethodsThe course consists of a mixture of lectures (8h), practical’s (36h),
computer sessions (3h) and individual study.
Method of AssessmentReports (50 %), oral presentation (50 %). A minimum grade of 5,0 is
required for each partial examination.
LiteratureLecture slides and experimental protocols. Any biochemistry textbook can
be used for repetition.
Target AudienceStudents in the Minor Biomolecular Sciences & Neurosciences, Track
Additional InformationPart of the Minor Biomolecular Sciences & Neurosciences, Track
This course is scheduled in the first half of period 2.
This minor course requires a minimum of 25 participants.
A lab coat is mandatory for the lab practical lab work.Participation in
the Portal Courses Experimental Cell Biology I and II is expected.
Recommended background knowledgeParticipation in the Portal Courses Experimental Cell Biology I and II.
|Language of Tuition||English|
|Faculty||Faculty of Science|
|Course Coordinator||dr. D. Bald|
|Examiner||dr. D. Bald|
dr. D. Bald
ing. H.W.J. Hakvoort
You need to register for this course yourself
Last-minute registration is available for this course.
|Teaching Methods||Computer lab, Lecture, Practical|