Course ObjectiveTo obtain a basic understanding of the functioning of living cells at
the molecular level, and introduction to qualitative and quantitative
methods applicable inside and outside the living cell. To learn to
design and execute studies where these methods are applied, and to
interpretate the results.
Specifically, the students will be trained in:
• The use and interpretation of information about cellular processes
such as energy metabolism, regulation of gene expression, oxygen damage,
apoptosis and signal transduction.
• The design and execution of simple experiments concerning gene
expression, signal transduction, growth and metabolic processes.
• Application of Metabolic Control Analysis and Flux Balance Analyses to
quantify distribution of
control and regulation of cellular processes by individual cellular
reactions and of flow distributions within cells.
• The use of existing computer models of molecular systems in the cell
to explore the behaviour of these systems.
Course Content• Introduction to Metabolic Control Analysis and its basis in enzyme
kinetics and of Flux Balance Analyses.
• Laws and practical applications of Metabolic Control Analysis, and
extension of this method to include gene expression.
• Modular kinetic analysis of cellular networks.
• Structure and function of bacterial and mitochondrial respiratory
• Regulation of gene expression during respiration in microorganisms.
• Use of a depository of mathematical models to explore reaction systems
in the cell.
• Control Analysis of signal transduction as a key in the understanding
Teaching MethodsLectures (16.5 contact hours); workgroups (3 day parts, obligatory);
computer practical (1.5 contact hours, obligatory); practical project in
the department of Molecular Cell Biology (depending on the project
ca. 20 contact hours, obligatory).
Method of AssessmentWritten exam (70%)
Entry RequirementsParticipation in the course “From Protein to Cell” (1st half of period
LiteratureA syllabus is available on the Canvas site.
Target AudiencePart of the minor Biomolecular Science and Neuroscience, track
Additional InformationFor the practical a laboratory coat will be supplied. After the course,
the coat will be sterilised.
Participation in the course "From Protein to Cell" (1st half of period
2) is compulsory.
Students who chose to participate in the computer practicals of Jacky
Snoep, please bring your own lap top.
|Language of Tuition||English|
|Faculty||Faculty of Science|
|Course Coordinator||dr. R.J.M. van Spanning|
|Examiner||dr. R.J.M. van Spanning|
prof. dr. H.V. Westerhoff
dr. J.R. Haanstra
dr. R.J.M. van Spanning
prof. dr. J.L. Snoep
prof. dr. B. Teusink
You need to register for this course yourself
Last-minute registration is available for this course.
|Teaching Methods||Study Group, Lecture, Computer lab, Practical|