From Protein to Cell

Course Objective

Study fundamental properties of proteins in a concerted theoretical and

practical approach. We will cover principles and applications of protein

manipulation, over-production, purification, handling, as well as the

function of antibiotics as protein inhibitors. In the laboratory practicals

selected features of key proteins will be studied. The design of

experiments to address a certain open question and understanding of

experimental protocols are an important aspects of this course.

Course Content

The course consists of a mixture of lectures, practicals (in groups), and

individual study. We will cover concepts/methods/techniques that you can

use to study a broad range of relevant questions, e.g: how to manipulate,

produce and purify a protein using bacteria and how to design an

experimental strategy.

Additional Information Teaching Methods

The course consists of a mixture of lectures, practical’s, and individual

study. In total 6 hours of lectures are planned, the majority of the time will

be spent for preparing, carrying out the practicals and evaluating the

obtained experimental results.

Method of Assessment

Written reports on the performed experiments (100%)

Literature

Lecture slides and experimental protocols. Any biochemistry textbook can
be used for repetition.

Additional Information Target Audience

Students in the Minor Biomolecular Sciences.

Additional Information

This course is scheduled in the second half (weeks 5-8) of period 2.

A lab coat is mandatory for the lab practical lab work. Prior

participation in the courses Experimental Cell Biology I and II is expected.

Recommended background knowledge

Participation in the Portal Courses Experimental Cell Biology I and II.