Experimental Cell Biology II

2019-2020

Course Objective

The student has an overview of advanced techniques in Cell Biology and
understands their working principle. The student is acquainted with
current open questions and on-going developments in Cell Biology.
The student can use the gained knowledge in Cell Biology for analysis
of experiments/experimental results, for the design of experiments to
address open questions, and for writing of a research proposal.
The student is able to argue scientifically using correct terminology.

Course Content

In-depth discussion of modern Cell Biology, with a particular focus on
current and emerging experimental techniques. Researchers in the field
present current state-of-the-art topics in Cell Biology.

Lecture topics include:
• Advanced -omic approaches, such as genomics,
proteomics, metabolomics and interactomics
• (Confocal) Microscopy and Live Cell Imaging, Protein (GFP) labeling
and
visualization techniques
• Proteomics and mass spectrometry
• Systems Biology
• Knock-out and RNAi techniques
• Cell biology of the synapse

The student will work out and submit a research proposal on a chosen
topic in Cell Biology (group work).

Teaching Methods

Lectures (28 h), work discussions related to the research proposal (6h)
self-study in groups to repeat lecture material and for research
proposal.

Method of Assessment

Written exam (2/3), research proposal (1/3). Next to a minimum combined
grade of 6.0 for whole course a minimum grade of 5.0 is required for the
written exam and for the literature assignment.

Entry Requirements

This course is to be taken together with the accompanying course
Experimental cell Biology I.

Literature

No book mandatory. Useful books are:
Alberts et al. Molecular Biology of the Cell (more extensive,
recommended for Biomolecular track)
Alberts et al. Essential Cell Biology (more concise, recommended for
Neurobiology track).
For the research proposal you will also work with scientific literature
relevant for the chosen topic (search/discuss in small groups).

Additional Information

Compulsory portal course of the minor Biomolecular Sciences and
Neurosciences.
This minor course requires a minimum of 25 participants.
This course is offered in weeks 5-8 of period 1 and is to be taken
together with the course
Experimental cell Biology I (in week 1-4 of period 1).

Guestlecture:
Dr. J. van Buul (Sanquin). Basic (first and
second year level) courses in Cell Biology,
participation in Experimental Cell Biology I.

Recommended background knowledge

Basic (first and second year level) courses in Cell Biology,
participation in Experimental Cell Biology I.

General Information

Course Code AB_1048
Credits 6 EC
Period P1
Course Level 300
Language of Tuition English
Faculty Faculty of Science
Course Coordinator dr. D. Bald
Examiner dr. D. Bald
Teaching Staff dr. D. Bald
dr. K.W. Li
dr. ir. A.H. de Boer
prof. dr. ir. E.J.G. Peterman
dr. J.P. van Ulsen
prof. dr. B. Teusink
dr. A.M. van der Sar
drs. C.P. Kuijl
prof. dr. H.V. Westerhoff

Practical Information

You need to register for this course yourself

Last-minute registration is available for this course.

Teaching Methods Study Group, Lecture