From Protein to Cell

2019-2020

Course Objective

Final attainment:
The student understands:
• Principles and applications of protein over-expression, purification,
structure, function, and inhibition as well as the function of
antibiotics as protein inhibitors.
• General principles concerning design and carrying out experiments
related to protein science.
The student can:
Apply protein bio-chemistry methods (CRISPR-Cas, protein
over-expression, affinity chromatography, spectrophotometry,
fluorescence methods, gel electrophoresis, activity tests).

Course Content

The course consists of a mixture of lectures, practicals (in groups),
and individual study. We will cover concepts/methods/techniques that you
can use to study a broad range of relevant questions, e.g:
• How can I manipulate a protein using CRISPR/Cas?
• How can I produce a protein using bacteria?
• How can I purify a protein?
• How can I investigate the effect of an inhibitor/antibiotic on a
target protein?
• How can I investigate the effect of an inhibitor/antibiotic on a
target cell?
• How can I design my experimental strategy?
• Which factors I have to think about to make my experiment
successful?

Teaching Methods

The course consists of a mixture of lectures (8h), practical’s (36h),
and individual study.

Method of Assessment

Reports (50 %), oral presentation (50 %). A minimum grade of 5,0 is
required for each partial examination.

Literature

Lecture slides and experimental protocols. Any biochemistry textbook can
be used for repetition.

Target Audience

Students in the Minor Biomolecular Sciences & Neurosciences, Track
Biomolecular Sciences.

Additional Information

Part of the Minor Biomolecular Sciences & Neurosciences, Track
Biomolecular Sciences.
This course is scheduled in the first half of period 2.
This minor course requires a minimum of 25 participants.
A lab coat is mandatory for the lab practical lab work.Participation in
the Portal Courses Experimental Cell Biology I and II is expected.

Recommended background knowledge

Participation in the Portal Courses Experimental Cell Biology I and II.

General Information

Course Code AB_1052
Credits 6 EC
Period P2
Course Level 300
Language of Tuition English
Faculty Faculty of Science
Course Coordinator dr. D. Bald
Examiner dr. D. Bald
Teaching Staff dr. D. Bald
ing. H.W.J. Hakvoort

Practical Information

You need to register for this course yourself

Last-minute registration is available for this course.

Teaching Methods Computer lab, Lecture, Practical