Current Topics in Molecular and Cellular Toxicology


Course Objective

The course aims to give a general introduction to the use of cell
cultures, in particular induced pluripotent stem cells (iPSC), and their
application for toxicology and personalized medicine

Course Content

Subjects of this course/caput are optional and may vary, depending on
the rest of the program and/or the interest of the BSc-student(s). In
close cooperation with the teaching coordinator, several topics will be
chosen, focusing on induced pluripotent stem cells (iPSC) and
using stem cells for assessment of toxicity.

Detailed description:
Studying the effects of pharmaceuticals in human cell culture systems,
offers a number of advantages over animal experiments, including the use
of human material and the possibility to use mechanistic approaches to
understand the mechanism of action or mechanism of toxicity of a
compound. In particular the use of iPSC opens exciting new avenues, as
they provide unlimited material, are of non-cancerous origin, and can be
collected/generated from individual living patients. iPSC can be
generated from somatic cells, e.g. from skin fibroblast or blood cells,
and in the last decade, many patient-specific iPSC have been made,
including cells that carry SNPs or mutations in drug transporters or
metabolizing enzymes. These cells can then be differentiated into target
cells that are important in toxicology, including cells types of the
liver, kidney, heart and intestine, and employed in toxicology studies.
Topics addressed in this course include, general cell culture
techniques, iPSC reprogramming and differentiation strategies, and a
basic understanding in developmental biology and physiology of the
target organs known to be involved in toxicity. In addition, strengths
and limitations of iPSC for their potential application of studying
toxicity and personalized medicine will be discussed.

Teaching Methods

This course consists of a series of lectures as well as discussion
sessions of recent literature. For this, the students are expected to do
a literature search and to prepare a presentation on a selected topic.

Method of Assessment

An assignment on a selected topic in form of a short presentation or
report will count for 70% of the mark. A test on the lectures will count
for 30% of the mark. Both need to be passed to pass the course.
Whether assignment work is performed by each student individually or in
groups will depend on the number of students enrolled.


Starting course materials will be provided by the tutors, including
guidelines and selected papers and book chapters. The students are
expected to use this material as a start and as guidance to conduct
their own literature search.

Target Audience


Additional Information

The course, including the assignment and test will be held in English.

Recommended background knowledge

Cellulaire Biochemie
Bio-analyse en farmaceutische toxicologie
Farmacokinetiek & ADME-processen

General Information

Course Code XB_0010
Credits 6 EC
Period P6
Course Level 300
Language of Tuition English
Faculty Faculty of Science
Course Coordinator dr. A. Wilmes
Examiner dr. A. Wilmes
Teaching Staff dr. A. Wilmes
prof. dr. P. Jennings

Practical Information

You need to register for this course yourself

Last-minute registration is available for this course.

Teaching Methods Lecture
Target audiences

This course is also available as: