Vak nog zonder naam

2019-2020
Dit vak wordt in het Engels aangeboden. Omschrijvingen kunnen daardoor mogelijk alleen in het Engels worden weergegeven.

Doel vak

Final attainment:
The student understands:
• Principles and applications of protein over-expression, purification,
structure, function, and inhibition as well as the function of
antibiotics as protein inhibitors.
• General principles concerning design and carrying out experiments
related to protein science.
The student can:
Apply protein bio-chemistry methods (CRISPR-Cas, protein
over-expression, affinity chromatography, spectrophotometry,
fluorescence methods, gel electrophoresis, activity tests).

Inhoud vak

The course consists of a mixture of lectures, practicals (in groups),
and individual study. We will cover concepts/methods/techniques that you
can use to study a broad range of relevant questions, e.g:
• How can I manipulate a protein using CRISPR/Cas?
• How can I produce a protein using bacteria?
• How can I purify a protein?
• How can I investigate the effect of an inhibitor/antibiotic on a
target protein?
• How can I investigate the effect of an inhibitor/antibiotic on a
target cell?
• How can I design my experimental strategy?
• Which factors I have to think about to make my experiment
successful?

Onderwijsvorm

The course consists of a mixture of lectures (8h), practical’s (36h),
and individual study.

Toetsvorm

Reports (50 %), oral presentation (50 %). A minimum grade of 5,0 is
required for each partial examination.

Literatuur

Lecture slides and experimental protocols. Any biochemistry textbook can
be used for repetition.

Doelgroep

Students in the Minor Biomolecular Sciences & Neurosciences, Track
Biomolecular Sciences.

Overige informatie

Part of the Minor Biomolecular Sciences & Neurosciences, Track
Biomolecular Sciences.
This course is scheduled in the first half of period 2.
This minor course requires a minimum of 25 participants.
A lab coat is mandatory for the lab practical lab work.Participation in
the Portal Courses Experimental Cell Biology I and II is expected.

Aanbevolen voorkennis

Participation in the Portal Courses Experimental Cell Biology I and II.

Algemene informatie

Vakcode AB_1052
Studiepunten 6 EC
Periode P2
Vakniveau 300
Onderwijstaal Engels
Faculteit Faculteit der Bètawetenschappen
Vakcoördinator dr. D. Bald
Examinator dr. D. Bald
Docenten dr. D. Bald
ing. H.W.J. Hakvoort

Praktische informatie

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