Doel vak

The aim of this course is to get acquainted with the performance of
pharmacological assays and molecular biological techniques in the
laboratory. This course is linked to the course 'Molecular
Pharmacology' (XB_43001), addressing how drugs act on (patho)
physiological processes and pharmacological parameters.

Inhoud vak

To study the pharmacological parameters of drugs cellular model systems
(mammalian cells) expressing drug targets of interest will be used.
During the practical work students will perform ligand-receptor
interaction studies, functional studies to determine ligand potency and
efficacy (e.g. G protein signaling, beta-arrestin recruitment) and
antagonistic properties of compounds (biosensor assay). Moreover, they
will also get acquainted with the use of molecular biological
techniques. This practical work will be accompanied by background
lectures (molecular pharmacology XB-43001), exercises to determine
pharmacological parameters and data analysis and reporting.

Onderwijsvorm

• Laboratory instructions
• Assignments

Toetsvorm

- Practical skills
- Measurement reports
- Theoretical and practical excercises

Vereiste voorkennis

Before starting this course, the student must have passed the course
Cellulaire Biochemie (X_430624).

Literatuur

Rang & Dale’s Pharmacology 8th edition ISBN 978-0-7020-5362-7

Doelgroep

2nd year students Pharmaceutical Sciences

Overige informatie

This course needs to be taken together with the course Molecular
Pharmacology (XB_43001).
Attending excercise classes and practical work is mandatory.


Lecturers:
dr. Henry Vischer
dr. Marco Siderius