Systems Biology in Practice

2019-2020
Dit vak wordt in het Engels aangeboden. Omschrijvingen kunnen daardoor mogelijk alleen in het Engels worden weergegeven.

Doel vak

The aim of the course is to get acquainted with the interdisciplinary
approach of experimental microbial physiology, transcriptome analysis
and proteome analysis. Students will learn how information obtained by
experiments at the level of cellular behaviour, genetic profile and
enzymatic make-up can be combined in order to get insight in the
mechanisms underlying regulation and adaptation of microbial organisms.
Students will be introduced to the basic techniques and principles of
microbial physiology, transcriptome analysis, massspectrometry and data
analysis.

Inhoud vak

Throughout the course, the same experimental material will be used.
Microorganisms will be cultured under a set of different conditions in
the group Molec Microbial Phys and their physiology will be quantified
in terms of metabolic fluxes. Transcriptome analysis of the cells will
show how and to what extent the environment affects the metabolic
network at the genetic level. Finally the same cells will be analyzed at
the level of their protein composition by a proteomic analysis and by
enzyme activity assays in vitro. Lectures on more general subjects of
Microbiol Systems Biology will be organized to give the students a
flavor of practice and application.
The content of the course can be summarized:
o Theory and practice growth of bacterial growth, flux analysis,
biochemical assays;
o Theory and practice of genetic tools, gene expression,
transcriptomics;
o Theory and practice of mass spec and proteomics;
o Data analysis, protein identification, systems biology of micro
organisms

Onderwijsvorm

o Lecture
o Practicals

Toetsvorm

Written thesis, written lecture abstracts and oral presentation.

Literatuur

Literature:
o Lecture contents

Algemene informatie

Vakcode XM_0079
Studiepunten 6 EC
Periode P4
Vakniveau 400
Onderwijstaal Engels
Faculteit Faculteit der Bètawetenschappen
Vakcoördinator dr. ir. K.A. Feenstra
Examinator dr. ir. H.C.J. Hoefsloot
Docenten dr. ir. K.A. Feenstra

Praktische informatie

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Werkvormen Werkcollege, Hoorcollege, Computerpracticum, Practicum