Biomolecular Screening

2019-2020
Dit vak wordt in het Engels aangeboden. Omschrijvingen kunnen daardoor mogelijk alleen in het Engels worden weergegeven.

Doel vak

To learn, understand and work with modern analytical chemistry in the
life sciences to identify proteins. More specifically, you will learn
ways to find biologically active proteins in mixtures, purify them and
finally identify them using proteomics techniques.

Inhoud vak

During this course the potential of modern analytical and biological
screening techniques used in bioactivity screening of bioactive proteins
will be discussed. The emphasis will be on finding bioactive proteins in
complex biological samples by LC-MS in combination with post-column
bioassays. Protein identification strategies using bottom-up proteomics
proteomics approaches will be focused on during this course. You will
learn how to find biologically active proteins in complex mixtures and
know how to identify these proteins by their (partial) amino acid
sequence. Sample treatment and advanced sample preparation techniques
will play an important part in this as well as LC-MS, bioassays and
database searches with the proteomics data obtained. We will work with
natural extracts such as snake venoms as our complex biological samples,
which contain potential biopharmaceutical candidate proteins.

Onderwijsvorm

The course starts with a thorough explanation of the course and its
contents. Then we will start with the practical work. Before and in
between the practical work, material will be provided to the students on
the theoretical background, the research topic, the techniques used, and
the methodologies applied during the practical work. In case of 8 or
more participants, lectures will be given to provide the needed
background to perform the practical work and prepare the assignment. In
case of less than 8 participants, some or all of the needed background
material will be provided as self study material before the course
starts. In this case, the teachers will personally assist and guide
students through the background material provided and go through study
material that individual students have questions about. Relevant
material and literature will be provided via Canvas. All students will
(besides their practical report) work on an assignment related
to a topic on bioactivity profiling in a biopharmaceutical setting.
Teams of students (teams of two students are anticipated) will be formed
for the practical research work and for the assignment.

Toetsvorm

The mark given for the assignment will constitute 50% of the
final mark. The mark for the practical work & report also constitutes
50% of the
final mark. This mark is given for the insights shown, motivation and
other relevant issues such as presence during the course, practical
course report, safety considerations and practical results obtained Both
marks have to be at least 5.5.
Presence during the practical course days is obligatory.

Vereiste voorkennis

Basic knowledge of biochemistry, separation sciences and mass
spectrometry.

Literatuur

Literature to study is mainly from e-books (chapters) and from academic
papers/reviews. All literature needed can be found in the course
documents on Canvas. Tutorials will be given by the course
supervisors during the practical work. All PowerPoint lectures will be
placed on Canvas at least one day before each lecture. All PDF e-
book chapters and other literature (e.g. academic research papers and
reviews) can already be found on Canvas.

Doelgroep

Master phase practical course

Algemene informatie

Vakcode X_432542
Studiepunten 3 EC
Periode P4
Vakniveau 400
Onderwijstaal Engels
Faculteit Faculteit der Bètawetenschappen
Vakcoördinator dr. J. Kool
Examinator dr. J. Kool
Docenten dr. J. Kool

Praktische informatie

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